How to cite item

Effects of tetrandrine on free intracellular Ca2+ in isolated rat brain cells

   
@article{APS8800,
	author = {Wei-Zhong Zeng and Tian-Pei Liu},
	title = {Effects of tetrandrine on free intracellular Ca2+ in isolated rat brain cells},
	journal = {Acta Pharmacologica Sinica},
	volume = {14},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {Using Ca(2+)-sensitive fluorescent indicator, Fura-2/AM, intracellular free Ca2+ ([Ca2+]i) was measured. Resting [Ca2+]i was 221 +/- 18 nmol.L-1 in the presence of Ca2+ 1.3 mmol.L-1 in Hank's solution. Tetrandrine (Tet) 30 mumol.L-1 had no effect on the resting [Ca2+]i when the extracellular Ca2+ were 0-2 mmol.L-1. In the presence of extracellular Ca2+ 1.3 mmol.L-1, Tet (1-100 mumol.L-1) concentration-dependently inhibited the high extracellular K(+)-induced [Ca2+]i elevation, with an IC50 value of 8.2 mumol.L-1 (95% confidence limits: 1.9-32.9 mumol.L-1). Low concentrations of Tet (1-10 mumol.L-1) did not alter the norepinephrine-induced [Ca2+]i elevation. Tet 30 mumol.L-1 depressed norepinephrine 10 mumol.L-1 induced [Ca2+]i elevation by 42%. The results suggested that Tet inhibited the Ca2+ influx through voltage-dependent ionic channels and, at high concentrations, through receptor-operated ionic channels in the brain cells.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8800}
}