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Enzyme kinetics of cefotaxime hydrolysed by beta-lactamases extracted from Gram-negative bacilli

   
@article{APS8670,
	author = {Shu-Ya Yan and Jia-Tai Li},
	title = {Enzyme kinetics of cefotaxime hydrolysed  by  beta-lactamases  extracted from Gram-negative bacilli},
	journal = {Acta Pharmacologica Sinica},
	volume = {9},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {The  enzyme  kinetics  of cefotaxime and cefoperazone against plasmid-and chromosomal-mediated beta-lactamases were studied with double-wavelength and double-beam spectrophotometer. beta-Lactamases were extracted from Gram-negative bacilli  and identified by isoelectric focusing method with the special reagent nitrocefin. Michaelis constant (Km), maximal velocity (Vmax) and inhibition constant (Ki) values were obtained from Lineweaver-Burk plots. The results showed that cefotaxime was more stable than cefaloridine and cefoperazone against the hydrolysis of (3-lactamases. Vmax of cefotaxime to TEM-1, SHV-1, P99 enzymes were},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/8670}
}