%0 Journal Article %T Effects of bleomycins on synchronized cells by centrifugal elutriation %A Wu De-Zheng %A Keng Peter %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Effects of bleomycins on synchronized cells by centrifugal elutriation %K %X Chinese hamster ovary (CHO) cells and human oat cell lung carcinoma (ME-MAR) cells were synchronized by centrifugal elutriation. The study of bleomycins (BLM) cytotoxicity to CHO cells showed that G2M phase was sensitive to BLM, S phase intermediate, and G1 phase resistant. In synchronized MEMAR cells the late S phase was sensitive, G2M phase intermediate, and G1 phase resistant to BLM toxicity. DNA distribution patterns measured by flow cytometry showed that a significant G2 block was obtained in CHO cells exposed to BLM 4-15 vg/ml. BLM also prevented cell passage from S to G2 phase. A similar G2 block was found in MEMAR cells. However, no S phase accumulation was found up to BLM 15 vg/ml. %U http://www.chinaphar.com/article/view/8597 %V 9 %N 2 %P 170-174 %@ 1745-7254