@article{APS8465,
author = {Ya-si Xu and Jian-guo Feng and Dan Zhang and Bo Zhang and Min Luo and Dan Su and Neng-ming Lin},
title = {S-allylcysteine, a garlic derivative, suppresses proliferation and induces apoptosis in human ovarian cancer cells in vitro },
journal = {Acta Pharmacologica Sinica},
volume = {35},
number = {2},
year = {2016},
keywords = {},
abstract = {Ya-si XU1, 3, Jian-guo FENG3, Dan ZHANG1, Bo ZHANG2, 3, Min LUO1, Dan SU1, 3, *, Neng-ming LIN2, 3, *
1College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310022, China; 2Laboratory of Clinical Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China; 3Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China
Aim: To investigate the effects of S-allylcysteine (SAC), a water-soluble garlic derivative, on human ovarian cancer cells in vitro.
Methods: Human epithelial ovarian cancer cell line A2780 was tested. Cell proliferation was examined with CCK-8 and colony formation assays. Cell cycle was analyzed with flow cytometry Cell apoptosis was studied using Hoechst 33258 staining and Annexin V/PI staining with flow cytometry. The migration and invasion of A2780 cells were examined with transwell and wound healing assays. The expression of relevant proteins was detected with Western blot assays.
Results: SAC (1−100 mmol/L) inhibited the proliferation of A2780 cells in dose- and time-dependent manners (the IC50 value was approximately 25 mmol/L at 48 h, and less than 6.25 mmol/L at 96 h). Furthermore, SAC dose-dependently inhibited the colony formation of A2780 cells. Treatment of A2780 cells with SAC resulted in G1/S phase arrest and induced apoptosis, accompanied by decreased expression of pro-caspase-3, Parp-1 and Bcl-2, and increased expression of active caspase-3 and Bax. SAC treatment significantly reduced the migration of A2780 cells, and markedly decreased the protein expression of Wnt5a, p-AKT and c-Jun, which were the key proteins involved in proliferation and metastasis.
Conclusion: SAC suppresses proliferation and induces apoptosis in A2780 ovarian cancer cells in vitro.
Keywords: ovarian cancer; anticancer drug; garlic; S-allylcysteine; proliferation; apoptosis; cancer metastasis
This work was supported by the Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents (2010-190-4) and the Zhejiang Key Laboratory of Diagnosis and Treatment Technology on Thoracic Oncology (Lung and Esophagus) (2012-02).
* To whom correspondence should be addressed.
E-mail lnm1013@163.com (Neng-ming LIN); smilesusu2003@126.com (Dan SU)
Received 2013-07-08 Accepted 2013-10-16},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8465}
}