@article{APS8386,
author = {Xuan-fen ZHANG and Shu-zhong GUO and Kai-hua LU and Hui-yuan LI and Xiang-dong LI and Lin-xi ZHANG and Li YANG},
title = {Different roles of PKC and PKA in effect of interferon-gamma on proliferation and collagen synthesis of fibroblasts},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {10},
year = {2016},
keywords = {},
abstract = {AIM:
To study the signal roles of protein kinase C (PKC) and protein kinase A (PKA) in the influence of interferon-gamma (IFN-gamma) on proliferation and collagen synthesis of fibroblasts derived from hypertrophic scar (HS-FB) and normal skin (NS-FB).
METHODS:
HS-FB and NS-FB were cultured and passaged in Dulbecco modified Eagles medium (DMEM). Activity of PKC and PKA were assayed by transferring phosphorus (32P) into substrate after treatment with IFN-gamma 1000 kU/L at 10, 30, 60, and 120 min. Cell proliferation was determined with MTT assay. The collagen synthesis was measured with [3H]proline incorporation and Type III pre-collagen was determined with radioimmunoassay.
RESULTS:
After exposure to IFN-gamma 1000 kU/L for 30 min, PKC activity of HS-FB and NS-FB increased from 2.57 +/- 0.14 and 2.13 +/- 0.12 nmol x min(-1) x g(-1) of control to 3.75 +/- 0.19 and 3.36 +/- 0.16 nmol x min(-1) x g(-1), respectively (P < 0.05). After exposure to IFN-gamma 1000 kU/L for 60 and 120 min, PKA activities of HS-FB increased gradually from 0.82 +/- 0.04 nmol x min(-1) x g(-1) of control to 1.03 +/- 0.05 and 1.23 +/- 0.06 nmol x min(-1) x g(-1), respectively (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8386}
}