@article{APS8343,
author = {Li-hong LIU and Xiao-kui WANG and Yuan-dong HU and Jian-lei KANG and Li-li WANG and Song LI},
title = {Effects of a fatty acid synthase inhibitor on adipocyte differentiation of mouse 3T3-L1 cells},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {8},
year = {2016},
keywords = {},
abstract = {AIM:
To investigate the influence of C75, a fatty acid synthase inhibitor, on adipocyte differentiation.
METHODS:
Mouse 3T3-L1 preadipocytes were induced to differentiation by insulin, isobutylmethylxanthine, and dexamethasone. Oil red O staining was performed and activity of glycerol-3-phosphate dehydrogenase (GPDH) was measured. The level of peroxisome proliferators-activated receptor gamma (PPARgamma) mRNA was assayed by semi-quantitative reverse transcription PCR.
RESULTS:
C75 blocked the adipogenic conversion in a dose-dependent manner and the inhibitory effects occurred both in the early phases (48 h) and in the latter phases (8 d) of the process. Treatment with C75 for 8 d induced more decrease in lipid content than 48 h (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8343}
}