TY - JOUR AU - Yi Fu-xian AU - Liu Wen-lan AU - Chen Li-wei AU - Zeng Shan AU - Guo Zhao-gui PY - 2016 TI - Hydrolysis of extracellular adenine nucleotides by cultured bovine endocardial endothelial cells. JF - Acta Pharmacologica Sinica; Vol 20, No 8 (August 1999): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - AIM: To characterize the ATP diphosphohydrolase (apyrase) of bovine endocardial endothelial cells, and to compare ecto-adeninenucleotidase activity between bovine endocardial and aortic endothelial cells (BEEC and BAEC). METHODS: The nucleotide was analyzed by reversed phase HPLC and apyrase activity was assayed by inorganic phosphate release. RESULTS: Apyrase inhibitors, both NaN3 10 mmol.L-1 and NaF 20 mmol.L-1, inhibited BEEC apyrase activity by 51% and 38%, respectively. The inhibitor for Na+/K(+)-ATPase, ouabain, did not affect the enzyme activity. Edetic acid 5 mmol.L-1 completely inhibited the enzyme activity. H2O2 0.5 mmol.L-1 downregulated BEEC apyrase activity in a time-dependent manner. The apyrases activities in BAEC were higher than those in BEEC, while the ecto-AMPase activity in BAEC was much weaker than that in BEEC. CONCLUSION: BEEC have NaN3- and NaF-sensitive, ouabain-insensitive apyrase activity. BEEC had high ecto-AMPase activities, and low apyrases activities as compared with BAEC. UR - http://www.chinaphar.com/article/view/8242