%0 Journal Article %T Effects of Cordyceps militaris extract on angiogenesis and tumor growth %A YOO Hwa-seung %A SHIN Jang-woo %A CHO Jung-hyo %A SON Chang-gue %A LEE Yeon-weol %A PARK Sang-yong %A CHO Chong-kwan %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Effects of Cordyceps militaris extract on angiogenesis and tumor growth %K %X AIM: To evaluate the effects of Cordyceps militaris extract (CME) on angiogenesis and tumor growth. METHODS: Human umbilical vein endothelial cells (HUVEC), HT1080, and B16-F10 cells were used. DNA fragment, angiogenic related gene expressions (MMPs, bFGF, VEGF, etc), capillary tube formation, wound healing in vitro, tumor growth in vivo were measured. RESULTS: CME inhibited growth of HUVECs and HT1080 (P<0.01). CME 100 and 200 mg/L reduced MMP-2 gene expression in HT1080 cells by 6.0 % and 22.9 % after 3-h and 14.9 % and 32.8 % after 6-h treatment. CME did not affect MMP-9 gene expression in B16-F10 melanoma cells. CME 100 and 200 mg/L also reduced bFGF gene expression in HUVECs by 22.2 % and 41.3 %. CME inhibited tube formation of endothelial cells in vitro and in vivo. CME repressed the growth of B16-F10 melanoma cells in mice compared with control group (P<0.05). CONCLUSION: CME has antiangiogenetic properties. %U http://www.chinaphar.com/article/view/8197 %V 25 %N 5 %P 657-665 %@ 1745-7254