TY  - JOUR
AU  - XU Tong-hui
AU  - ding Jun
AU  - SHI Yu-liang
PY  - 2016
TI  - Toosendanin increases free-Ca(2+) concentration in NG108-15 cells via L-type Ca(2+) channels
JF  - Acta Pharmacologica Sinica; Vol 25, No 5 (May 2004): Acta Pharmacologica Sinica
Y2  - 2016
KW  - 
N2  - AIM:  To examine if toosendanin (TSN) affects intracellular free-Ca(2+) concentration ([Ca(2+)](i)) in neuroblastoma pluglioma hybrid cells (NG108-15 cells).  METHODS:  The [Ca(2+)](i) was determined by laser-scanning confocal microscopic imaging technique in which Fluo-3 was used as Ca(2+) indicator.  RESULTS:  TSN induced an increase in resting [Ca(2+)](i) and in high K(+)-evoked Ca(2+) transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca(2+)](i) was dose-dependent and disappeared in CdCl(2-), nifedipine-containing or Ca(2+)-free solution, and appeared after washing out the Ca(2+) channel blockers or adding Ca(2+).  CONCLUSION:  TSN increased [Ca(2+)](i) in differentiated NG108-15 cells. The [Ca(2+)](i) enhancement was due to the influx of extracellular Ca(2+) and related to L-type Ca(2+) channels.
UR  - http://www.chinaphar.com/article/view/8188