@article{APS8130,
author = {Yuan-Xiang Tao and Yun-Qing Li and Zhi-Qi Zhao},
title = {Synaptic interaction between GABAergic terminals and substance P receptor-positive neurons in rat spinal superficial laminae},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {10},
year = {2016},
keywords = {},
abstract = {\"AIM:
To identify synaptic relationship between gamma-aminobutyric acid (GABA)-containing terminals and substance P receptor-positive neurons in the superficial dorsal horn of rat spinal cord.
METHODS:
A combination of preembedding immunoperoxidase staining for substance P receptor with postembedding immunogold staining for GABA was employed.
RESULTS:
Intense substance P receptor-expressing neurons were concentrated in medial half of the lamina I and the outer lamina II of the spinal cord. Under electron microscopy, substance P receptor immunoreactivity was found mainly in perikarya and dendrites, and deposited along neuronal membranes, in Golgi complex, in rough endoplasmic reticulum and on the surface of mitochondria with patch- or granular-like staining. The double-labeling studies revealed that substance P receptor-expressing dendrites received symmetric synaptic contacts from axonal terminals labeled with immunogold particles indicating GABA. GABA also co-localized with substance P receptors in the dendrites.
CONCLUSION:
The synaptic contact between GABA-containing terminal and substance P receptor-expressing neuron provided important morphological evidence for previous pharmacological studies concerning antinociceptive function of GABA in the spinal cord.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/8130}
}