%0 Journal Article %T Tetrahydroprotoberberines inhibit lipid peroxidation and scavenge hydroxyl free radicals %A Jin Xi-Lu %A Shao Yue %A Wang Ming-Jie %A Chen Li-Juan %A Jin Guo-Zhang %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Tetrahydroprotoberberines inhibit lipid peroxidation and scavenge hydroxyl free radicals %K %X "AIM: To study the effects of tetrahydroprotoberberines (THPB) on rat liver and brain lipid peroxidation (LPO) and oxygen free radicals generation. METHODS: The malondialdehyde (MDA) levels in rat brain and liver homogenates, induction of MDA by Fe(2+)-Vit C in mitochondria, OH. generation by Fenton reaction, and O2.- generation by pyrogallol oxidation were observed in vitro. RESULTS: (1) THPB lowered the MDA contents in the liver homogenate and mitochondria, and the IC50 values of l-THPB-18 and l-stepholidine (SPD) in the liver mitochondria were 3.1 and 12.7 mumol.L-1 respectively. SPD decreased the MDA contents in the brain homogenate and mitochondria with IC50 values of 102 and 35.0 mumol.L-1 respectively. (2) THPB scavenged OH., and the IC50 values of l-THPB-18 and SPD were 0.21 and 3.8 mumol.L-1 respectively, but no effect on O2.- was observed. CONCLUSION: THPB could reduce the MDA contents and scavenge OH. and THPB-18 was the most potent amongst them." %U http://www.chinaphar.com/article/view/8030 %V 21 %N 5 %P 477-480 %@ 1745-7254