@article{APS7998,
author = {Xun Guo and Li-Wei Chen and Wen-Lan Liu and Zhao-Gui Guo},
title = {High glucose inhibits expression of inducible and constitutive nitric oxide synthase in bovine aortic endothelial cells},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {4},
year = {2016},
keywords = {},
abstract = {\"AIM:
To investigate the effects of high glucose on the expression of nitric-oxide synthase (NOS) in cultured bovine aortic endothelial cells (BAEC).
METHODS:
BAEC were cultured and passaged in normal glucose (NG) 5.5 mmol.L-1, high glucose (HG) 25 mmol.L-1, or high osmolarity (glucose 5.5 mmol.L-1 + mannitol 19.5 mmol.L-1, Mann-BAEC), lipopolysaccharides (LPS)-induced nitric oxide (NO) production was assessed by Griess reaction. The expression of inducible NOS (iNOS) and constitutive NOS (ecNOS) was determined by Western blot.
RESULTS:
At a concentration range from 0.5 to 2 mg.L-1, LPS stimulated NO production in NG-BAEC in a concentration-dependent manner. NO production reached the peak level at LPS 1 mg.L-1. HG inhibited NO production, when compared with NG- and Mann-BAEC (nitrite mumol.L-1: HG-BAEC 43 +/- 8, vs NG-BAEC 71 +/- 11, Mann-BAEC 70 +/- 9, n = 4 experiments, P < 0.01). iNOS expression was decreased by 39.9% and 39.3%, and ecNOS by 28% and 24% respectively in HG-BAEC, when compared with NG- or Mann-BAEC. However, no marked difference was observed in the LPS-induced NO production and the expression of iNOS and ecNOS between NG- and Mann-BAEC.
CONCLUSIONS:
Inhibition of BAEC NO production by HG was mainly due to a decreased expression of NOS protein.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7998}
}