TY - JOUR AU - Fu Li-Ying AU - Li Yang AU - Cheng Lan AU - Zhou Hong-Yi AU - Yao Wei-Xing AU - Xia Guo-Jin AU - Jiang Ming-Xing PY - 2016 TI - Effect of sea anemone toxin anthopleurin-Q on sodium current in guinea pig ventricular myocytes JF - Acta Pharmacologica Sinica; Vol 22, No 12 (December 2001): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Aim: To investigate the effects of a sea anemone toxin anthopleurin-Q (AP-Q) isolated from Anthopleura xanthogrammica on sodium current (INa) in isolated guinea pig ventricular myocytes. Methods: Single myocytes were dissociated by enzymatic dissociation method. INa was recorded using whole-cell patch-clamp technique. Results: AP-Q (3 - 300 nmol/L) increased INa in a concentration-dependent manner. The EC50 value for increasing INa was 104 nmol/L (95 % confidence range: 78 - 130 nmol/L). AP-Q 300 nmol/L shifted the I-V curve to the leftward, changed the membrane potential of half maximal activation to more negative potential from (-36.3 +/- 2.3) mV to (-43 +/- 3) mV (n = 6, P Conclusion: AP-Q has a stimulating effect on I(Na) with slowing the inactivation course of INa. UR - http://www.chinaphar.com/article/view/7916