TY - JOUR AU - ZHANG Ting AU - LI Jun-ling AU - XIN Jian AU - MA Xiao-chao AU - TU Zeng-hong PY - 2016 TI - Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones JF - Acta Pharmacologica Sinica; Vol 25, No 2 (February 2004): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - AIM: To compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones (FQ). METHODS: Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and levofloxacin (LELX) were tested by comet assay and photodynamic DNA strand breaking activity under the different conditions of UVA irradiation. RESULTS: In comet assay, photogenotoxicity was evident at SPFX 1 mg/L, LFLX 5 mg/L, and CPFX 5 mg/L, and LELX 10 mg/L. In photodynamic DNA strand-breaking activity, SPFX and LFLX induced the conversion of the supercoiled form into the nicked relaxed form at 10-50 micromol/L, while CPFX at 25 micromol/L and LELX at 50 micromol/L. CONCLUSION: There were good correlations between the two methods to detect DNA damage induced by phototoxicity of fluoroquinolones. Photodynamic DNA strand breaking activity was a good method to detect DNA damage induced by photogenotoxicity of fluoroquinolones as well as comet assay. UR - http://www.chinaphar.com/article/view/7857