@article{APS7838,
author = {Yun-bi LU and Ming WU and Han-liang ZHOU},
title = {Changes of phospholipase D activity of rat peritoneal mast cells in degranulation},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM:
To study the changes of phospholipase D (PLD) activity of actively sensitized rat peritoneal mast cells (RPMC) in degranulation.
METHODS:
Degranulation of RPMC was determined by measurement of beta-hexosaminidase release. PLD activity assay was carried out by measurement of PLD product, choline, with chemiluminescent oxidation of luminol.
RESULTS:
Actively sensitized RPMC challenged with ovalbumin (0.5-8 mg/L for 120 s, 4 mg/L for 15-120 s) resulted in significant activation of PLD accompanied with the increment of beta-hexosaminidase release. PLD activity of sensitized RPMC was increased by more than 2-fold compared with that of unsensitized RPMC which contained low levels of PLD activity [(35+/-13) pmol choline/min in 10(6)cells], but beta-hexosaminidase releases of the sensitized cells were as low as spontaneous releases. After challenge with ovalbumin 4 mg/L for 120 s, PLD activity of sensitized RPMC was increased to (155+/-43) pmol choline/min in 10(6)cells and beta-hexosaminidase release was also elevated significantly (4.5-fold of spontaneous release, n=6, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7838}
}