@article{APS7826,
author = {Chi WANG and Tian-hui CHANG and Xin-hua ZHANG and Huai-liang WANG},
title = {Effects of dl-praeruptorin A on nucleus factor-kappaB and tumor necrosis factor-alpha expression in ischemia-reperfusion hearts},
journal = {Acta Pharmacologica Sinica},
volume = {25},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM:
To study the effects of dl-praeruptorin A (Pd-Ia) on nucleus factor-kappaB (NF-kappaB) activativity and tumor necrosis factor-alpha (TNF-alpha) expression in ischemia-reperfusion (I/R) myocardium.
METHODS:
Langendorff's isolated rat heart was subjected to a 10-min ischemia followed by a 30-min reperfusion. NF-kappaB activity in nucleus was analyzed by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). TNF-alpha level in cytoplasm was measured by radioimmunoassay. Infiltration of neutrophils was observed using Hematoxylin-Eosin staining under optical microscope.
RESULTS:
Pd-Ia 1.0 micromol/L with 30-min preventive perfusion decreased NF-kappaB activity from 0.98+/-0.13 to 0.65+/-0.17 (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7826}
}