@article{APS7814,
author = {Yu-hong Niu and Xue-yi Yang and Wei-sheng Bao},
title = {Protective effects of Ginkgo biloba extract on cultured rat cardiomyocytes damaged by H2O2},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {7},
year = {2016},
keywords = {},
abstract = {AIM: To investigate the influence of Ginkgo biloba extract (GbE) on cardiomyocytes damaged by H2O2.
METHODS: Cultured rat cardiomyocytes were divided into 3 groups randomly: control group; H2O2 (2.5 mmol.L-1) group; H2O2 2.5 mmol.L-1 + GbE 150 mg.L-1 group. The cardiomyocytes were cultured in MEM (Eagle's) at 37 degrees C in the presence of 5% CO2 for 4 h. Lactate dehydrogenase (LDH) was assayed by colorimetric method. Lipid peroxidation was determined by measuring thiobarbituric acid-reactive substances. Ultrastructure was viewed under transmission electron microscope.
RESULTS: Compared with the control group, LDH leakage and malondialdehyde (MDA) content increased in H2O2 group, LDH increased from (2166 +/- 247) U.L-1 to (5180 +/- 648) U.L-1, MDA increased from (3.5 +/- 0.2) nmol/10(6) cells to (7.2 +/- 0.4) nmol/10(6) cells (P < 0.01). The ultrastructure was damaged seriously. GbE inhibited the increase of LDH leakage and MDA content induced by H2O2. In this group, LDH decreased from (5180 +/- 648) U.L-1 to (3496 +/- 386) U.L-1, MDA decreased from (7.2 +/- 0.4) nmol/10(6) cells to (4.8 +/- 0.9) nmol/10(6) cells (P < 0.01). Ultrastructure of cells was also protected by GbE.
CONCLUSION: GbE protected the cardiomyocyte against H2O2 injury, the protective action was attributed to its antiperoxidative effect.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7814}
}