@article{APS7795,
author = {Jin Li and Xin Li and Gang Pei and Bo-yi Qin},
title = {Influence of agmatine in adaptation of cAMP signal transduction system of opiate receptors},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {7},
year = {2016},
keywords = {},
abstract = {AIM: To observe attenuative effects of agmatine on opiate desensitization and substance dependence.
METHODS: Guanosine 5'-O-(3-[35S] thiotriphosphate) ([35S]GTTP) binding and cellular cyclic AMP (cAMP) level were determined by radioligand binding assay and radioimmunoassay in NG108-15 cells, respectively.
RESULTS: Agmatine increased stimulative action of opioids on [35S]GTTP binding by about 35% and inhibitory effects of opioids on cellular cAMP concentration by about 114.3% in NG108-15 cells pretreated with opioids. On the other hand, it also inhibited cAMP over-shooting by 214.9% of morphine substance dependent cells precipitated by naloxone compared with that of control. These effects of agmatine were antagonized by idazoxan in a concentration-dependent manner.
CONCLUSION: Agmatine reversed the formative process of adaptation in cAMP signal transduction cascade.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7795}
}