@article{APS7668,
author = {Biao-xin Chai and Zhong-ming Tang and Xiu-wen Liu and Min Tu},
title = {Tissue distribution of recombinant human tumor necrosis factor alpha derivative in mice},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {4},
year = {2016},
keywords = {},
abstract = {AIM:
To study the tissue distribution and its mechanism of a new recombinant tumor necrosis factor alpha derivative (rhTNF alpha Da) in mice.
METHODS:
125I-rhTNF alpha Da was prepared by Iodogen method. Tissue distribution of 125I-rhTNF alpha Da in mice was studied by determining radioactivity of tetrachloroacetic acid (TCA)- precipitable fraction in tissues. The isolated heart-lung perfusion study using 125I-rhTNF alpha Da perfusate was carried out to study the distribution characteristics of 125I-rhTNF alpha Da in lung.
RESULTS:
Except for thyroid, AUC of the TCA-precipitable 125I-rhTNF alpha Da in tissues was highest in lung, which was 12.2-fold of that in serum, while concentrations in other tissues were all lower than that in serum. Perfusion study in vitro revealed that the concentration of radio-labeled peptide in lung was higher than that in perfusate. On the contrary, level in heart was much lower than that in perfusate. The overall distribution of 125I-rhTNF alpha Da in lungs showed rapidly equilibratory, dose-dependent, saturable, competitive, and highly affinitive, with Kd 47.6 pmol.L-1 and Bmax 348 fmol.g-1 (lung tissue).
CONCLUSION:
The specific distribution of rhTNF alpha Da in lungs was its distinctive characteristics.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7668}
}