TY - JOUR AU - Zhang Yu-Qiu AU - Gao Xiu AU - Ji Guang-Chen AU - Huang Ya-Lin AU - Wu Gen-Cheng PY - 2016 TI - Expression of 5-HT2A receptor mRNA in rat nucleus raphe magnus neurons after peripheral inflammation JF - Acta Pharmacologica Sinica; Vol 22, No 10 (October 2001): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Aim: Examine the expression of 5-HT2A receptor mRNA and co-localization of 5-HT2A receptor mRNA with 5-HT immunoreactivity in nucleus raphe magnus (NRM) neurons following carrageenan inflammation. Methods: In situ hybridization (ISH) and combining fluorescent ISH with immunofluorescent histochemical double staining technique were used in the present study. Results: 5-HT2A receptor mRNA was expressed in moderate level in NRM neurons. Following carrageenan inflammation, the expression of 5-HT2A receptor mRNA in NRM was greatly increased. The peak occurred at 3 - 8 h and then there was a clear decrease at 24 h after injection of carrageenan. Only a few 5-HT2A receptor mRNA and 5-HT immunoreactive double labeled cells were observed in NRM, and the number of the double labeled cells had no obvious change following carrageenan inflammation. Conclusion: The synthesis of 5-HT2A receptor was increased in non-serotonin rather than serotonin neurons of NRM during peripheral inflammation. UR - http://www.chinaphar.com/article/view/7647