@article{APS7647,
author = {Yu-Qiu Zhang and Xiu Gao and Guang-Chen Ji and Ya-Lin Huang and Gen-Cheng Wu},
title = {Expression of 5-HT2A receptor mRNA in rat nucleus raphe magnus neurons after peripheral inflammation},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: Examine the expression of 5-HT2A receptor mRNA and co-localization of 5-HT2A receptor mRNA with 5-HT immunoreactivity in nucleus raphe magnus (NRM) neurons following carrageenan inflammation.
Methods: In situ hybridization (ISH) and combining fluorescent ISH with immunofluorescent histochemical double staining technique were used in the present study.
Results: 5-HT2A receptor mRNA was expressed in moderate level in NRM neurons. Following carrageenan inflammation, the expression of 5-HT2A receptor mRNA in NRM was greatly increased. The peak occurred at 3 - 8 h and then there was a clear decrease at 24 h after injection of carrageenan. Only a few 5-HT2A receptor mRNA and 5-HT immunoreactive double labeled cells were observed in NRM, and the number of the double labeled cells had no obvious change following carrageenan inflammation.
Conclusion: The synthesis of 5-HT2A receptor was increased in non-serotonin rather than serotonin neurons of NRM during peripheral inflammation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7647}
}