@article{APS7478,
author = {Jun-Li Cao and Yin-Ming Zeng and Li-Cai Zhang and Jun Gu and Hui-Fen Liu and Wen-Hua Zhou and Guo-Dong Yang},
title = {NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {6},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effects of nitric oxide (NO) on activation of the rat spinal cord neurons during naloxone-precipitated morphine withdrawal.
Methods: Fos immunocytochemistry, NADPH-d histochemistry, Fos/NADPH-d double-labeling, intrathecal injection, antisense oligonucleotides (AS-ONs) techniques, and RT-PCR were used.
Results: Acute administration of naloxone and chronic administration of morphine did not change the expression of Fos protein and NADPH-d positive neurons, and there was no expression of Fos/NADPH-d double-labeled neurons in the spinal cord of rats. Morphine withdrawal increased the expression of Fos protein, NADPH-d positive, and Fos/NADPH-d double-labeled neurons, and they were observed in all the laminae of the rat spinal cord. Intrathecal injection of nNOS antisense oligonucleotides (nNOS-AS) inhibited the increase of Fos protein and NMDA(1A)R mRNA expression in the rat spinal cord during morphine withdrawal and decreased the scores of morphine withdrawal symptoms. The effect of nNOS-AS was greater than that of eNOS-AS. There was no effect in nNOS sense oligonucleotides (nNOS-S) group.
Conclusion: NO mediated the increase of Fos protein and NMDA1A R mRNA expression in the rat spinal cord during morphine withdrawal.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7478}
}