@article{APS7450,
author = {Sheng-nan Li and Manfred Brater and Klaus Andreas},
title = {Nicardipine inhibits N-type calcium channels in dbcAMP-differentiated neuroblastoma x glioma hybrid cells (NG 108-15 cells).},
journal = {Acta Pharmacologica Sinica},
volume = {20},
number = {2},
year = {2016},
keywords = {},
abstract = {AIM:
To investigate the possibility of dihydropyridine inhibition of N-type calcium channels.
METHODS:
Effects of nifedipine and nicardipine on the high K(+)-induced intracellular Ca2+ concentration ([Ca2+]i) increase were studied by measuring [Ca2+]i using the fluorescent indicator Fura-2.
RESULTS:
Pretreatment of cells with nifedipine 50 mumol.L-1 inhibited the high K(+)-induced [Ca2+]i transient by about 60% (n = 3); however, pretreatment of cells with nicardipine 10 mumol.L-1 completely prevented the high K(+)-evoked [Ca2+]i increase in dibutyryl cyclic AMP (dbcAMP)-differentiated NG 108-15 cells (n = 5). The high K(+)-induced [Ca2+]i increase was mediated by L- and N-type voltage-sensitive calcium channels (VSCC) in NG 108-15 cells.
CONCLUSION:
Nicardipine at micromolar range inhibited both L- and N-type VSCC in dbcAMP-differentiated NG 108-15 cells whereas nifedipine mainly inhibited L-type calcium channels.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7450}
}