@article{APS7390,
author = {Yan Shu and Ze-Neng Cheng and Zhao-Qian Liu and Lian-Sheng Wang and Bing Zhu and Song-Lin Huang and Dong-Sheng Ou-Yang and Hong-Hao Zhou},
title = {Interindividual variations in levels and activities of cytochrome P-450 in liver microsomes of Chinese subjects},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To compare the level and/or activity of several important cytochrome P-450 (CYP) enzymes in liver microsomes prepared from different Chinese subjects.
Methods: Individual CYP contents, including CYP1A2, CYP2C9, and CYP3A4, in liver microsomes of 17 Han, 17 Zhuang, and 8 Miao subjects were determined by using Western blot analysis and densitometric scanning. The substrates for measuring the activity of individual CYP in vitro included phenacetin, tolbutamide, debrisoquine, and omeprazole.
Results: There was a large interindividual variability in the content and activity of CYP1A2, 2C9 and 3A4. And the activity of CYP2D6 also varied greatly between individual samples. CYP3A4 (32 %) is the most abundant CYP in Chinese liver microsomes, and the levels of CYP2C9 (19 %) and CYP1A2 (16 %) were also considerable. No clear ethnic, sex- and age-related differences in individual CYP content and catalytic activity were detected in 42 Chinese liver samples, except that there were somewhat ethnic and sex-related differences in the content and activity of CYP1A2. Good correlation between enzyme protein content and activity was found for CYP1A2, 2C9 and 3A4.
Conclusion: Our results may provide useful information for the study of drug metabolism by liver microsomes in Chinese.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7390}
}