@article{APS7389,
author = {Shen Yang and Xue-Mei Zhang and Ming-Hua Jiang},
title = {Inhibitory effect of melittin on Na+,K+-ATPase from guinea pig myocardial mitochondria},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effect of melittin (Mel) of Na+,K+-ATPase activity and it's kinetic mode of action on guinea pig myocardial mitochondria.
Methods: Effect of Mel on heart mitochondrial Na+,K+-ATPase activity was determined with colorimetry method.
Results: Mel inhibited Na+,K+-ATPase in a concentration and time dependent manner, IC50 was 2.60 micromol/L. Kinetic studies of interaction between Mel and K+, Na+, ATP revealed that inhibitory effect of Mel was competitive with K+, but not with Na+ and ATP.
Conclusion: Mel polypeptide potently inhibits Na+,K+-ATPase, possibly by binding to the K+ site.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7389}
}