@article{APS7382,
author = {Ya-Feng Sun and Fang-Ming Tang and Yun-Min Ding and You-Ting Chen and Guang-Yi Zhang and Guo-Zhang Jin},
title = {Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To study the effects of dopamine depletion on the phosphorylation level, intracellular distribution, and mRNA expression of DARPP-32 in the ischemic striatum and to elucidate the mechanisms underlying the ischemic injury.
Methods: A complex model of SN lesioning with 6-OHDA to deplete dopamine and four vessels occlusion for inducing forebrain ischemia was constructed in rats. DARPP-32 was investigated with autoradiogram, immunohistochemistry and in situ hybridization.
Results: The [32P]phosphate incorporation of DARPP-32 was reduced in vitro following ischemia. However, the [32P]phosphate incorporation, the numbers of positive neurons, and mRNA expression of DARPP-32 were increased in SN lesioning plus ischemic rats with denervated striatum.
Conclusion: Dopamine depletion reduced the DARPP-32 phosphorylation in vivo following ischemia, and protected DARPP-32 immunoreactivity and mRNA expression level against the reduction induced by ischemia.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7382}
}