@article{APS7368,
author = {Lan Sun and Ling-Ling Weng and Hu Zheng and Jing-Sheng Liu},
title = {Effects of XW630 on cell proliferation, iNOS activity, and cGMP content in human osteoblast-like cell line TE85},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {3},
year = {2016},
keywords = {},
abstract = {\"AIM:
To study the effects of 2-[3-estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) in human osteoblast-like cell line TE85.
METHODS:
[3H]Thymidine incorporation and cell count for cell proliferation, radioimmunoassay for cyclic GMP (cGMP) content, and monitoring the conversion of [3H]arginine for inducible nitric-oxide synthase (iNOS) activity assay.
RESULTS:
After treatment with XW630 for 48 h, [3H]thymidine incorporation and cell numbers increased by 62.7% and 96.9%, respectively. NG-monomethyl-L-arginine (L-NMMA, an NOS inhibitor) induced a concentration-dependent inhibitory effect on the proliferation after treatment for 48 h. The inhibitory effect was prevented partially by XW630 (1.0 nmol.L-1). After treatment with XW630 for 12-48 h, iNOS activity and cGMP concentration increased in time-dependent manners.
CONCLUSION:
XW630 stimulated cell proliferation, enhanced iNOS activity and cGMP content in human osteoblast-like cell line TE85.\"},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7368}
}