@article{APS7231,
author = {Wen-Ya Wang and Ming-Tao Li and Rong-Biao Pi and Peng-Xin Qiu and Xing-Wen Su and Sui-Zhen Lin and Guang-Mei Yan},
title = {Antagonistic action of caffeine against LY294002-induced apoptosis in cerebellar granule neurons},
journal = {Acta Pharmacologica Sinica},
volume = {21},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: To study the effect of caffeine on apoptosis induced by inhibition of 1-phosphatidylinositol 3-kinase in cerebellar granule neurons.
METHODS: Cerebellar granule neurons culture, agar gel electrophoresis, and stress-activated protein kinase (SAPK)/c-Jun N-terminal protein kinase (JNK) assay kit to measure SAPK/JNK activity.
RESULTS: LY294002 evoked apoptosis concentration-dependently in cerebellar granule neurons. But death resulting from LY294002 was prevented by caffeine in a concentration-dependent manner. The survival effect of caffeine was not affected by inhibitors of ryanodine-sensitive Ca2+ release, nor was it inhibited by L-type channel blockers and N-methyl-D-aspartate (NMDA) receptor blocker. In addition, RP-cAMP, H89, and KN62 were not able to inhibit the protective effect of caffeine. Phosphorylation of c-Jun was necessary for the induction of apoptosis induced by LY294002 in cerebellar granule neurons. But caffeine directly inhibited the activation of JNK and decreased phospho-c-Jun in granule neurons.
CONCLUSION: Caffeine inhibited the activation of JNK and decreased the phosphorylation of c-Jun to protect granule neurons from LY294002-induced apoptosis.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7231}
}