@article{APS7220,
author = {Ke-Jian Zhu and Qian-Yun Shen and Min Zhang and Ulrich Mrowietz},
title = {Effects of calcitriol and its analogues on interaction of MCP-1 and monocyte derived dendritic cells in vitro},
journal = {Acta Pharmacologica Sinica},
volume = {22},
number = {1},
year = {2016},
keywords = {},
abstract = {Aim: To study the effect of calcitriol [1,25(OH)2D3] and its analogues on the interaction of monocyte chemoattractant protein-1 (MCP-1) and in vitro generated monocyte-derived dendritic cells (MoDC).
Methods: MoDC were obtained by differentiating monocytes in exposure to GM-CSF and IL-4 for 5 d. mRNA expression of MCP-1 and its receptors were analyzed by RT-PCR, and protein production of MCP-1 by ELISA and migratory ability of MoDC in response to MCP-1 by a micromultiwell chemotaxis chamber assay.
Results: MoDC can express MCP-1 mRNA, and secret a low level of MCP-1 protein and has the ability to migrate to MCP-1 in corresponding to its expression of MCP-1 receptors. Calcitriol and its analogues with the same affinity to vitamin D receptor up-regulated the gene expression of both MCP-1 and its receptors, enhanced MCP-1 protein production and promoted the migratory ability of MoDC to MCP-1.
Conclusion: The interaction of DC and MCP-1 found in this study may suggest a possible auto-regulatory role between DC and MCP-1 and the modulatory effect of calcitriol and its analogues on DC and MCP-1 might provide an understanding of their positive role in tumors.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7220}
}