@article{APS7129,
author = {Jun MA and Peng-Ling ZHU and Jing-Wen XIE and Zheng-Ping JIA and Jin-Sheng YANG},
title = {Restricted-access media high pressure liquid chromatography vs fluorescence polarization immunoassay for analysis of carbamazepine in human plasma.},
journal = {Acta Pharmacologica Sinica},
volume = {23},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: To compare restricted-access media high performance liquid chromatographic
(RAM-HPLC) method with fluorescence polarization immunoassay (FPIA) for analysis
of carbamazepine (CBZ) in human blood.
METHODS: An RAM-HPLC method was established for the determination of CBZ in
plasma.
RESULTS: The two methods do not need sample clean-up prior to analysis and they
have almost 100 % recovery and good reproducibility. There is a good correlation
between the CBZ concentration in venous plasma samples determined by FPIA and
that in both venous and fingertip plasma samples obtained by RAM-HPLC, the
correlation coefficients being 0.989 and 0.995, respectively. It is shown by
t-test that the data sets of venous and fingertip plasma samples given by
RAM-HPLC are consistent with each other but significantly different from the
results obtained by FPIA.
CONCLUSION: Both direct injection RAM-HPLC and FPIA may be applied in determining
CBZ in therapeutic drug monitoring (TDM). FPIA is well-suited to the routine TDM.
RAM-HPLC is more useful in TDM related research and especial cases.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/7129}
}