TY  - JOUR
AU  - Wang Ji-Cheng
AU  - Qian Bei-Li
PY  - 2016
TI  - Detection of DNA damage in peripheral lymphocytes by 7 compounds using comet assay
JF  - Acta Pharmacologica Sinica; Vol 18, No 5 (September 1997): Acta Pharmacologica Sinica
Y2  - 2016
KW  - 
N2  - AIM: To detect the DNA single strand breaks (SSB) in peripheral lymphocytes of  mice, rats, and human induced by hydrogen peroxide (H2O2), ethyl methane  sulphonate (EMS), dimethylnitrosamine (DMNA), mitomycin C (MMC), benzo (a) pyrene  (BaP), cyclophosphamide (CP), and 2-aminofluorene (2-AF).  METHODS: Alkaline single cell microgel electrophoresis assay in vitro (comet  assay).  RESULTS: All were positive with 2 exceptions: EMS (0.97 mmol.L-1) in mice and MMC  (30 mumol.L-1) in mice and human. The lowest concentrations detectable were H2O2   (1 mumol.L-1), EMS (0.48 mmol.L-1), BaP (5.0 mumol.L-1), CP (2.0 mmol.L-1), MMC  (10 mumol.L-1), DMNA (27.3 mmol.L-1), and 2-AF (62.5 mumol.L-1). CP, BaP, and  2-AF were positive only in the presence of metabolic activation system.  CONCLUSION: H2O2, DMNA, BaP, CP, and 2-AF induce SSB in peripheral lymphocytes of  mice, rats, and human detected by comet assay, whereas MMC induces SSB only in  rats, and EMS in rats and human lymphocytes.
UR  - http://www.chinaphar.com/article/view/6975