@article{APS6930,
author = {Xue-Bin Tao and Wan-Hai Li},
title = {Assay of ditiocarb sodium and its methyl ester in mouse plasma by column-switching HPLC},
journal = {Acta Pharmacologica Sinica},
volume = {17},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM: To establish a column-switching high pressure liquid chromatograpy (CSHPLC) with direct injection for determination of ditiocarb sodium (DTC) and its methyl ester (DTC-Me) in mouse plasma.
METHODS: After automated online pretreating column enrichment and clear-up, DTC-Me was flushed and chromatographed on an ordinary reversed-phase analytical column, and monitored by UV at lambda absorption = 276 nm. DTC was methylated before determination.
RESULTS: No potential interfering peaks were identified. The calibration curves of both analytes were linear within the range of 0.1-150 mg.L-1. The correlation coefficients of DTC and DTC-Me were 0.9998 and 0.9995, respectively. The detection limit was 25 micrograms.L-1 and the coefficient of variation in the assay was within 7% for both compounds. The average recoveries were in the range of 95.28 -100.06%. A typical application was presented for mouse after i.v. DTC 50 mg.kg-1.
CONCLUSION: The rapid CSHPLC method with direct injection can be used for the study of pharmacokinetics of DTC and DTC-Me.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6930}
}