@article{APS6929,
author = {Jian-Hua Qi and Lu Zhang and Jun Wang and Pei-Kun Gu and Zheng-Jun Jin and Ming-Zhi Huang and Xin-Ming Wang},
title = {Effect of captopril on intracellular pH in vascular smooth muscle cells},
journal = {Acta Pharmacologica Sinica},
volume = {17},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM: To observe the effect of captopril (Cap) on intracellular pH (pHi) in aortic smooth muscle cells (ASMC).
METHODS: Cultured ASMC derived from rat and rabbit aortae were loaded with the fluorescent dye BCECF and pHi was determined using digital image processing method.
RESULTS: The pHi of untreated SHR and WKY rats were 7.37 +/- 0.29 and 7.19 +/- 0.31, respectively. Oral Cap decreased pHi (7.11 +/- 0.26, P < 0.05) and exaggerated pHi response to angiotensin II (Ang-II, 0.1 mumol.L-1) in ASMC of SHR rats vs WKY rats (0.14 +/- 0.05 vs 0.21 +/- 0.05 pH units, P < 0.01). Cap in vitro had no effect on Ang-II induced intracellular alkalinization in ASMC of rabbits.
CONCLUSION: Oral Cap inhibits Na+(-)H+ exchange activity in ASMC of SHR rats.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6929}
}