@article{APS6895,
author = {Bumsup Lee and Ter-Hsin Chen and Walter H Hsu},
title = {Oxytocin-stimulated insulin release in a clonal beta-cell line RINm5F: involvement of phospholipase C-dependent and -independent pathways},
journal = {Acta Pharmacologica Sinica},
volume = {17},
number = {5},
year = {2016},
keywords = {},
abstract = {AIM: To study the mechanisms underlying oxytocin (Oxy)-induced insulin release.
METHODS: In a clonal pancreatic beta-cell line, RINm5F cells.
RESULTS: Oxy increased insulin release and [Ca2+]i in a concentration-dependent manner. Oxy-induced insulin release was not altered by pretreatment with pertussis toxin (PT). U-73122 (2-8 mumol.L-1), a phospholipase C (PLC) inhibitor, concentration-dependently inhibited Oxy-induced increases in [Ca2+]i with IC50 value of 2.8 +/- 0.2 mumol.L-1. In addition, U-73122 diminished the Oxy-induced increase in intracellular concentration of inositol 1, 4, 5-triphosphate (IP3). U-73122 at 8 mumol.L-1 totally abolished the Oxy-induced increases in [Ca2+]i and IP3; however it reduced the Oxy-induced increase in insulin release only by 36% and 63% in the monolayer and suspended cell preparations, respectively.
CONCLUSION: Oxy increases insulin release through both PLC and non-PLC mediated signal transduction mechanisms.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6895}
}