@article{APS6812,
author = {Xiu-Wu Zhang and Xi-Lin Niu and Zhao-Gui Guo},
title = {Estrogens induce apoptosis in mouse peritoneal macrophages},
journal = {Acta Pharmacologica Sinica},
volume = {18},
number = {3},
year = {2016},
keywords = {},
abstract = {AIM: To study whether estrogen might induce apoptosis in mouse peritoneal
macrophages (MPM).
METHOD: The MPM were isolated and incubated in culture medium containing
17-beta-estradiol, estrone, or equal volume of 100% ethanol as control. DNA
fragmentation was visualized by agarose gel electrophoresis.
RESULTS: 17-beta-Estradiol 0.01-1 mumol.L-1 or estrone 10-20 mumol.L-1 elicited
typical morphological apoptosis and DNA fragmentation in a
concentration-dependent manner in MPM. Staurosporine (Sta) 0.01 mumol.L-1,
cycloheximide (Cyc) 1 mg.L-1, and tamoxifen (Tam) 10 mumol.L-1 inhibited the DNA
fragmentation induced by 17-beta-estradiol 1 mumol.L-1 or estrone 20 mumol.L-1.
CONCLUSION: Estradiol and estrone induced apoptosis in MPM.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6812}
}