@article{APS6707,
author = {Chen Li and Feng Zhang and Hong Lin and Zhong-can Wang and Xin-jian Liu and Zhen-qing Feng and Jin Zhu and Xiao-hong Guan},
title = {Generation and characterization of the human neutralizing antibody fragment Fab091 against rabies virus},
journal = {Acta Pharmacologica Sinica},
volume = {32},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To transform the human anti-rabies virus glycoprotein (anti-RABVG) single-chain variable fragment (scFv) into a Fab fragment and to analyze its immunological activity.
Methods: The Fab gene was amplified using overlap PCR and inserted into the vector pComb3XSS. The recombinant vector was then transformed into E coli Top10F' for expression and purification. The purified Fab was characterized using SDS-PAGE, Western blotting, indirect ELISA, competitive ELISA, and the fluorescent antibody virus neutralization test (FAVN), respectively, and examined in a Kunming mouse challenge model in vivo.
Results: A recombinant vector was constructed. The Fab was expressed in soluble form in E coli Top10F'. Specific binding of the Fab to rabies virus was confirmed by indirect ELISA and immunoprecipitation (IP). The neutralizing antibody titer of Fab was 10.26 IU/mL. The mouse group treated with both vaccine and human rabies immunoglobulin (HRIG)/Fab091 (32 IU/kg) showed protection against rabies, compared with the control group (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6707}
}