@article{APS6683,
author = {Yang Yang and Qian Shi and Ze Liu and Ruo-jie Li and Peng-wei Pan and Yuan-yuan Hou and Wan-ge Lu and Gang Bai},
title = {The synergistic anti-asthmatic effects of glycyrrhizin and salbutamol},
journal = {Acta Pharmacologica Sinica},
volume = {31},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the efficacy of glycyrrhizin (GL) combined with salbutamol (SA) as an anti-asthma therapy.
Methods: Rat lung beta2-adrenergic receptor (β2-AR) mRNA level was measured by real-time RT PCR. Intracellular cAMP accumulation was evaluated with a reporter gene assay. An in vitro acetylcholine-induced guinea pig tracheal strip contraction model was used to test the relaxing effects of GL and SA. The anti-inflammatory effects of GL and SA were tested using tumor necrosis factor-α-induced NF-κB transcriptional activation reporter assay, I-κB Western blotting and interleukin-8 ELISA. An in vivo guinea pig asthma model was used to prove further the synergistic effect of GL and SA.
Results: GL (0.3 μmol/L) increased mRNA levels of β2-AR in vivo and the accumulation of cAMP in vitro. The combination of GL and SA also resulted in significant complementary anti-inflammatory effects via inhibition of NF-κB activation, degradation of I-κB and production of interleukin-8. A significant synergistic effect of the combination was detected both in vitro and in vivo in a guinea pig mode.
Conclusion: The results demonstrate that GL and SA have synergistic anti-asthmatic effects and offer the possibility of a therapeutic application of GL in combination with β2-AR agonists in the treatment of asthma.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6683}
}