@article{APS6608,
author = {Hong-liang Kong and Jian-ping Wang and Zhan-quan Li and Shu-mei Zhao and Jing Dong and Wei-wei Zhang},
title = {Anti-hypoxic effect of ginsenoside Rbl on neonatal rat cardiomyocytes is mediated through the specific activation of glucose transporter-4 ex vivo },
journal = {Acta Pharmacologica Sinica},
volume = {30},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: The aim of this study was to investigate whether Gs-Rbl relieves the CoCl2-induced apoptosis of hypoxic neonatal rat cardiomyocytes and in which the role of glucose transporter-4 (GLUT-4).
Methods: Gs-Rbl (0, 10, 50, 100, 200, 400, and 500 μmol/L), adenine 9-β-D-arabinofuranoside (ara A, 500 μmol/L; AMPK inhibitor) and wortmannin (0.5 μmol/L; PI3K inhibitor) only in combination with 200 μmol/L Gs-Rbl were administered in hypoxic cardiomyocytes, which were induced by 500 μmol/L CoCl2 for 12 h. Then, the apoptotic rate (AR), 2-[3H]-deoxy-D-glucose (2-[3H]-DG) uptake, and the expression of GLUT-4 (including in plasma membrane, PM), phospho-AMPKα (Thr172), AMPKα and Akt in cells were assayed.
Results: Compared with simple hypoxia (0 μmol/L Gs-Rbl), Gs-Rb1 greater than 10 μmol/L significantly decreased the apoptotic rate (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6608}
}