@article{APS6570,
author = {Hai-Bo Qiu and Jia-Qi Pan and Yong-Qiang Zhao and De-Chang Chen},
title = {Effects of dexamethasone and ibuprofen on LPS-induced gene expression of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung},
journal = {Acta Pharmacologica Sinica},
volume = {18},
number = {2},
year = {2016},
keywords = {},
abstract = {AIM: To study the kinetics of tumor necrosis factor alpha (TNF alpha),
interleukine-1 (IL-1 beta), and macrophage inflammatory protein-1 alpha (MIP-1
alpha) gene expression in rat lung after i.p. lipopolysaccharides (LPS) and the
effect of dexamethasone (Dex) and ibuprofen (Ibu) on the cytokines gene
expression.
METHODS: The amount of Evans blue in lung was measured by fluorescence method.
The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung were
assessed by slot blot analysis.
RESULTS: The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung
after i.p. LPS increased in a dose-dependent manner, and peaked at 2, 6, and 12
h, respectively. Both Dex 50 mg.kg-1 and Ibu 90 mg.kg-1 injected at 1 h before
i.p. LPS markedly decreased the content of Evans blue in lung at 1 h after i.p.
LPS. After Dex or Ibu pretreatment, the peak levels of TNF alpha, IL-1 beta, and
MIP-1 alpha mRNA decreased markedly compared with LPS alone.
CONCLUSION: The gene expression of TNF alpha, IL-1 beta, and MIP-1 alpha in rat
lung increased after i.p. LPS. Dex and Ibu prevented LPS-induced lung injury
through inhibiting the cytokines gene expression.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6570}
}