TY - JOUR AU - Dai De-Zai AU - An Lu-Fan AU - Huang Dan-Yang AU - Sun Rui-Yuan PY - 2016 TI - Orthogonal analysis of aggravating effects of alpha-, beta-agonists and leukocytes on reperfusion-induced arrhythmias and ventricular fibrillation in Langendorff's rat heart JF - Acta Pharmacologica Sinica; Vol 17, No 2 (March 1996): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - AIM: To study the effect of leukocyte (Leu), alpha-agonist (alpha-Ago), and beta-agonist (beta-Ago) on the arrhythmias induced by ischemia and reperfusion to determine which of the 3 factors was the most important one in exacerbating arrhythmias. METHODS: Arrhythmias were induced by the reduction and subsequent resumption of perfused flow in Langendorff's perfused rat hearts. Ventricular tachycardia (VT) and ventricular fibrillation (VF) were recorded on ECG, and the results were orthogonally analyzed. RESULTS: When Leu was present, the incidence of VF induced by ischemia-reperfusion was 80%. The incidence in Leu-depleted hearts was 20%, alpha-Ago and beta-Ago elevated it to 60% and 100%, respectively. The results by orthogonal analysis demonstrated Leu or alpha-Ago+ beta-Ago increased VF incidence. With regard to arrhythmias, arrhythmia score was remarkedly increased by all of 3 factors and various combinations except beta-Ago + Leu. CONCLUSION: Among these 3 factors, Leu was the most important one in facilitating reperfusion-induced arrhythmias. UR - http://www.chinaphar.com/article/view/6379