%0 Journal Article %T Effects of captopril and enalapril on intracellular Ca2+ in vascular smooth muscle cell %A Qi Jian-Hua %A Zhang Lu %A Wang Jun %A Wei Pei-Jing %A Gu Pei-Kun %A Jin Zheng-Jun %A Huang Ming-Zhi %A Wang Hong-Yuan %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Effects of captopril and enalapril on intracellular Ca2+ in vascular smooth muscle cell %K %X AIM: To determine whether angiotensin-converting enzyme inhibitors can affect Ca 2+ handling in cultured aortic smooth muscle cells (ASMC) directly. METHODS: Cultured ASMC derived from rat aorta were loaded with the intracellular Ca 2+ ([Ca]2+i) fluorescent indicator Fura 2-AM and digital image processing technique was used. RESULTS: Resting [Ca 2+ ]i was greater in ASMC from SHR vs WKY (P 2+ ]i increases were enhanced in ASMC of SHR vs WKY (220 +/- 6, 212 +/- 8, and 215 +/- 14 vs 199 +/- 6, 202 +/- 7, and 195 +/- 7 nmol.L-1, respectively). Captopril (Cap) and enalapril (Ena) had no inhibitory effect on KCl-, NE-, and Ang-induced [Ca 2+ ]i increases in ASMC of WKY. Cap and Ena inhibited KCl-, NE-, and Ang-increased [Ca 2+ ]i in ASMC of SHR (210 +/- 7, 194 +/- 6, and 201 +/- 6 nmol.L-1, respectively). Ena and nifedipine similarly decreased KCl-, NE-, and Ang-increased [Ca 2+ ]i. CONCLUSION: Cap blocked KCl-, NE-, and Ang-increased ([Ca 2+ ]i) via a voltage-dependent Ca 2+ channel of which function and specificity was altered in ASMC of SHR. %U http://www.chinaphar.com/article/view/6377 %V 17 %N 2 %P 142-145 %@ 1745-7254