@article{APS6376,
author = {Rui Fei and Yu Fei and Sheng Zheng and Yan-guang Gao and Hong-xia Sun and Xian-lu Zeng},
title = {Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion.
Methods: The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.
Results: p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.
Conclusions: p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6376}
}