@article{APS6297,
author = {Li-na Sun and Liao-liao Li and Zheng-bin Li and Ling Wang and Xiao-liang Wang},
title = {Protective effects of TREK-1 against oxidative injury induced by SNP and H 2 O 2 },
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: TREK-1 (TWIK-related K+ channel-1) is a 2-pore-domain K+ channel subtype. The present study investigated the role of TREK-1 in cell death induced by oxidative stress.
Methods: The cell viability of wild-type Chinese hamster ovary (CHO) and TREK-1-transfected CHO cells (TREK-1/CHO cells) was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence of sodium nitroprusside (SNP) or hydrogen peroxide (H2O2). Apoptosis of wild-type CHO and TREK-1/CHO cells was detected using Hoechst33342 staining.
Results: Both SNP and H2O2 caused dose- and time-dependent growth inhibition of wild-type CHO and TREK-1/CHO cells. Following a 12 h exposure to SNP, the 50% inhibition (IC50) values for wild-type CHO and TREK-1/CHO cells were calculated as 0.69 mmol/L and 1.14 mmol/L, respectively. The IC50 values were 0.07 mmol/L and 0.09 mmol/L in H2O2-treated wild-type CHO and TREK-1/CHO cells, respectively, following 12 h exposure to H2O2. Moreover, SNP/H2O2 induced less apoptosis in TREK-1/CHO cells than that in wild-type CHO cells (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6297}
}