TY - JOUR AU - Leung Yuk-Man AU - Kwan Chiu-Yin AU - Loh Tatt-Tuck PY - 2016 TI - Capacitative Ca 2+ entry in HL-60 cells: tetrandrine and SK & F 96365 as probes. JF - Acta Pharmacologica Sinica; Vol 17, No 2 (March 1996): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Agonist-activated Ca 2+ entry is important in many biological responses such as secretion and cell growth(1,2). In nonexcitable cells which have no voltage-operated Ca 2+ channels (VOCC), agonist-receptor interaction can trigger Ca 2+ entry across the plasmalemma via several entry pathways(1-3) (Fig 1): (A) channels which are intrinsic structures of the receptor (receptor-operated channels), (B) channels which are coupled to receptors via a G-protein (G-protein-operated channels), (C) channels which are activated by some second messengers (second-messenger-operated channels), and (D) channels which open upon intracellular nonmitochondrial Ca 2+ store depletion (Ca 2+ release-activated channels) resulting from inositol 1, 4, 5-trisphosphate-induced Ca 2+ release or inhibition of Ca 2+ re-uptake (see next section). Ca 2+ entry via the 4th type of channel, also known as "capacitative Ca 2+ entry" (CCE)[4], has aroused much interest in the past decade because of its intriguing nature as retrograde signalling. In this brief review, we present the evidence for and the possible biochemical processes involved in CCE. We also discuss the use of 2 novel Ca 2+ entry blockers: tetrandrine and SK&F 96365. Emphasis will be put on the human leukemic HL-60 cell line, a popular cell system for intracellular Ca 2+ homeostasis studies and also a model the signal transduction of which we have been investigating during the past few years. UR - http://www.chinaphar.com/article/view/6282