TY - JOUR AU - Zhou Xiao-Ping AU - Wu Xiao-Ping AU - Guan Bing-Cai AU - Li Zhi-Wang PY - 2016 TI - Modulatory effects of gonadorelin on GABA-induced depolarization and GABA-activated current in rat spinal ganglion neurons JF - Acta Pharmacologica Sinica; Vol 17, No 1 (January 1996): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - AIM: To explore the modulatory effects of gonadorelin on GABA-induced depolarization and GABA-activated current in membrane of rat primary sensory neurons. METHODS: Intracellular recordings and whole-cell patch clamp techniques were performed on neurons in rat spinal ganglia (SG) preparation and neurons freshly isolated from rat SG, respectively. Drugs were applied by superfusion and/or by bath application. RESULTS: In the majority of neurons GABA (10 mumol.L-1 -1 mmol.L-1) induced a depolarization, which was blocked by bicucullin (100 mumol.L-1). Pretreatment with gonadorelin (50 mumol.L-1) decreased the GABA-induced depolarization by 79 +/- 22% (n = 29), while gonadorelin elicited no effect or slight depolarization alone. In 6 of 11 cells, GABA-activated currents were also inhibited by pretreatment with gonadorelin (50 mumol.L-1), while in 5 of 11 cells, there was no change or a slight potentiation. CONCLUSION: Gonadorelin exerts an inhibitory effect on GABA-induced depolarization and GABA-activated current in the primary sensory neurons. UR - http://www.chinaphar.com/article/view/6246