@article{APS6081,
author = {Gao-de LI and Shu-qin LIU and Xiu-yu YE and Feng-yi QU},
title = {Detection of 54-kDa protein overexpressed by chloroquine-resistant Plasmodium berghei ANKA strain in pyronaridine-resistant P berghei ANKA strain},
journal = {Acta Pharmacologica Sinica},
volume = {16},
number = {1},
year = {2016},
keywords = {},
abstract = {AIM: A 54-kDa protein overexpressed by chloroquine-resistant (CR) Plasmodium berghei ANKA strain was first reported by us. This study is conducted to detect the protein in pyronaridine-resistant (PR) P berghei ANKA strain.
METHODS: Immunoblotting analysis and immunoelectron microscopy were used.
RESULTS: PR parasites, like CR parasites, mainly overexpressed 2 major bands of 37 (36-38) kDa and 16 (15-17) kDa which were considered to be 2 subunits of 54 (52-62) kDa protein. Three of 7 times of experiments showed a 54-kDa and a 96 (95-100) kDa bands. The proteins were localized to be mainly scattered in cytoplasm of trophozoites, schizonts, and merozoites of erythrocytic stage of P berghei. Some of them were distributed in cytoplasm of erythrocytes infected with parasites.
CONCLUSION: Both PR and CR parasites overexpressed the same proteins.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6081}
}