@article{APS6065,
author = {Ying-Bin Liang and Xiao-Ya Ma and Guo-Jie Wu and Li-Fang Wang and Deng-Ke Liu and Jian-Feng Xing and Geng-Sheng Zhao},
title = {Determination of m-nifedipine and its pharmacokinetic study in rabbits by high-pressure liquid chromatography},
journal = {Acta Pharmacologica Sinica},
volume = {13},
number = {2},
year = {2016},
keywords = {},
abstract = {A high-pressure liquid chromatographic method was developed for determination of m-nifedipine in plasma using a chemical bonded C-18 phase column (YWG-C18 10 microns, made in China) with nitrendipine as internal standard. To increase life of the YWG-C18 column a mixture of methanol and 5 mmol.L-1 phosphate buffer (70:30 vol/vol) was selected as mobile phase with a flow rate of 0.8 ml.min-1. The method was sensitive to m-nifedipine 3 ng.ml-1 plasma and the standard curve was linear from 10 to 1000 ng.ml-1 with correlation coefficient of 0.99. The within-day and day-to-day precisions (CV) of this method were 4.5% and 7.0%, respectively, with recoveries of 95-102% (10-1000 ng.ml-1). There was no interference with nifedipine, amiodarone, propranol, and verapamil. A pharmacokinetic study on m-nifedipine was carried out in 8 rabbits. A better computer fitted to a two-compartment model was observed using 3P87 program. The parameters obtained were as follow: Vc 6.3 L.kg-1, Cl 0.021 L.kg-1.min-1, T1/2 alpha 30 min, T1/2 beta 230 min, AUC 102 micrograms.min.ml-1.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/6065}
}