TY - JOUR AU - Zhang Yu AU - Ye Qi-xia AU - Liu Jian AU - Zhang Zheng-yan AU - Zhang Tan-mu PY - 2016 TI - Synergistic effect of probimane on anticancer cytotoxicity of doxorubicin in vitro JF - Acta Pharmacologica Sinica; Vol 15, No 1 (January 1994): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Using 3-(4,5-dimethythiazole)-2,5-diphenyltetrazolium bromide (MTT) method, the effect of probimane (Pro) on doxorubicin (Dox) cytotoxicity was studied. Pro 0.313, 0.625, and 1.25 micrograms.ml-1 potentiated cytotoxicity of Dox in Ehrlich ascites carcinoma (EAC) cells. Incubation of EAC cells with Dox 10 micrograms.ml-1 and Pro 116.5, 233, and 466 micrograms.ml-1 resulted in an increase in intracellular drug accumulation from 0.69 +/- 0.06 to 1.08 +/- 0.10 micrograms/10(7) cells. In S37-bearing mice, Pro 23.3, 46.6, and 116.5 micrograms.ml-1 enhanced the malondialdehyde (MDA) formation in tumor and liver mitochondria and decreased MDA formation in liver mitochondria. These results suggested that the increases of Dox accumulation and MDA formation in tumor cells by Pro might be the reasons for synergistic effect of Pro on Dox cytotoxicity UR - http://www.chinaphar.com/article/view/6028