@article{APS5899,
author = {Li-yang Cui and Shang-li Liu and Yue Ding and Dong-sheng Huang and Ruo-fan Ma and Wei-guo Huang and Bao-shan Hu and Qiu-hui Pan},
title = {IL-1beta sensitizes rat intervertebral disc cells to Fas ligand mediated apoptosis in vitro</em.},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {10},
year = {2016},
keywords = {},
abstract = {Aim: To determine the apoptotic effect of recombinant rat Fas Ligand on rat inter-vertebral disc cells pre-treated with IL-1beta in vitro, and the expression of Fas in cultured rat intervertebral disc cells.
Methods: Cells were isolated from the inner annulus fibrosus and transition zones of lumbar discs from Sprague-Dawley rats. The cells were grown in monolayer and divided in 5 treatment groups. IL-1beta (10 ng/mL), FasL (5, 20 ng/mL) with/without IL-1beta (10 ng/mL) pre-treatment was respectively added in Dulbecco's modified Eagle's medium and Ham's F-12 medium with 1% fetal bovine serum. After 32 h, the cells were stained with annexin V-FITC and propidium iodide to evaluate apoptosis using flow cytometry and to analysis transcription of Fas using RT-PCR.
Results: Compared with control group, FasL (20 ng/mL), IL-1beta (10 ng/mL)+FasL (5 ng/mL), and IL-1beta (10 ng/mL) +FasL (20 ng/mL) induced significant apoptosis of the disc cells (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5899}
}