TY - JOUR AU - Yang Zhong-wei AU - Li Dong-jie AU - Liu Chong AU - Han Ping AU - Yang Yi-li AU - Su Ding-feng AU - Shen Fu-ming PY - 2016 TI - Role of vascular K ATP channels in blood pressure variability after sinoaortic denervation in rats JF - Acta Pharmacologica Sinica; Vol 32, No 2 (February 2011): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Aim: To investigate the role of ATP-sensitive potassium (K ATP ) channels on blood pressure variability (BPV) in sinoaortic denervated (SAD) rats. Methods: SAD was performed on male Sprague-Dawley rats 4 weeks before the study. mRNA expression of Kir6.1, Kir6.2 and SUR2 in aorta and mesenteric artery was determined using real-time quantitative polymerase chain reaction, and confirmed at the protein level using Western blotting and laser confocal immunofluorescence assays. Concentration-response curves of isolated aortic and mesenteric arterial rings to adenosine and pinacidil were established. Effects of K ATP channel openers and blocker on BPV were examined in conscious SAD rats. Results: Aortic SUR2 expression was significantly greater, while Kir6.1 was lower, in SAD rats than in sham-operated controls. In contrast, in the mesenteric artery both SUR2 and Kir6.1 expression were markedly lower in SAD rats than controls. For both arteries, Kir6.2 expression was indistinguishable between sham-operated and SAD rats. These findings were confirmed at the protein level. Responses of the aorta to both adenosine and pinacidil were enhanced after SAD, while the mesenteric response to adenosine was attenuated. Pinacidil, diazoxide, nicorandil, and glibenclamide significantly decreased BPV. Conclusion: These findings indicate that expression of vascular K ATP channels is altered by chronic SAD. These alterations influence vascular reactivity, and may play a role in the increased BPV in chronic SAD rats. UR - http://www.chinaphar.com/article/view/5808